Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Fariq Fitri MSM
43400 Serdang
Malaysia
Research Article
A Rapid Liquid Chromatography Method for Determination of Gylphosate
in Crude Palm Oil with Fluorescence Detection
Author(s): Fariq Fitri MSM, Halimah Muhamad, Dzolkhifli Omar and Norhayu AsibFariq Fitri MSM, Halimah Muhamad, Dzolkhifli Omar and Norhayu Asib
A rapid and simple method for the determination of glyphosate in crude palm oil (CPO) was developed and validated using high performance liquid chromatography with fluorescence detector. Glyphosate was derivatized with 9-fluorenylmethylchloroformate (FMOC-Cl) and then separated using a C18 reverse phase column with potassium dihydrogen phosphate and acetonitrile as the mobile phase. A linear correlation was obtained for the concentration of glyphosate from 0.05-1.5 μg mL-1 with a correlation coefficient of 0.9998. The average recovery obtained for glyphosate ranged between 80% and 100% at five fortification levels with the relative standard deviation (RSD) of less than 3% of all cases. The limit of detection and limit of quantification for glyphosate were 0.05 and 0.1 μg/g, respectively. The method will facilitate palm oil trade through quality assurance in terms of g.. View More»
DOI:
10.4172/2157-7064.1000346