Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Fangjian Chen
Tianjin Key Laboratory for Industrial Biological Systems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences,
Tianjin 300308
China
Research Article
Identification of NaHCO3 Stress Responsive Proteins in Dunaliella salina
HTBS using iTRAQ-based Analysis
Author(s): Yue Zhao, Yuyong Hou, Zhiyong Liu, Shulin Chen and Fangjian ChenYue Zhao, Yuyong Hou, Zhiyong Liu, Shulin Chen and Fangjian Chen
Converting CO2 to HCO3- through CO32- as a carbon source for microalgae growth offers an alternative route for CO2 fixation. There, however, are concerns over algae growth inhibition caused by high HCO3- concentration. The exact mechanism of the microalgae response to NaHCO3 stress also needs further understanding. This study evaluated the effect of high HCO3- on enhancing growth of algae Dunaliella salina HTBS by identifying a series of differentially expressed proteins in responding to NaHCO3 stress using iTRAQ (isobaric tags for relative and absolute quantitation) assay. Results showed that HTBS featured with adaptability to broad HCO3- concentrations (25 to 70 g/L NaHCO3). The specific growth rate (OD750) reac.. View More»
DOI:
10.4172/jpb.1000399