Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Eva Cernuda-Morollon
Central University Hospital of Asturias,
Health Service of the Principality of Asturias, Oviedo
Spain
Research Article
Lysophosphatidylcholine Induces Vascular Smooth Muscle Cell
Membrane Vesiculation: Potential Role in Atherosclerosis through
Caveolin-1 Regulation
Author(s): Esther Serrano-Pertierra, Lorena Benavente, Miguel Angel Blanco-Gelaz, Jose Luis Fernandez-Martin, Carlos H Lahoz, Sergio Calleja, Carlos Lopez-Larrea and Eva Cernuda-MorollonEsther Serrano-Pertierra, Lorena Benavente, Miguel Angel Blanco-Gelaz, Jose Luis Fernandez-Martin, Carlos H Lahoz, Sergio Calleja, Carlos Lopez-Larrea and Eva Cernuda-Morollon
Background: Microparticles are present in low concentrations in normal plasma, but increase in several diseases including atherosclerosis, mainly through membrane activation processes or during apoptosis. The number of circulating microparticles has been proposed as a marker of subclinical atherosclerosis, but their potential role in its progression has not been fully characterized.
Methods: Microparticles released by vascular smooth muscle cells treated with lysophosphatidylcholine were isolated and their composition characterized by proteomic techniques. Some hits were confirmed by western blot in this and other cell lines involved in atherosclerosis. Platelet poor plasma was obtained from patients suffering from carotid stenosis (>70%) by venipuncture and subsequential centrifugation. Platelet poor plasma was analyzed by flow cytometry and the microparticle fraction was .. View More»
DOI:
10.4172/jpb.1000336