Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
El-Gamal RM
College of Pharmacy,
11942, Alkharj
Saudi Arabia
Research Article
Micellar Liquid Chromatography Coupled with Fluorometric Detection for the Determination of Terbinafine in Dosage Forms and Plasma
Author(s): Belal F, Sharaf El-Din MK, Eid MI and El-Gamal RMBelal F, Sharaf El-Din MK, Eid MI and El-Gamal RM
A newly developed micellar liquid chromatographic analysis technique was utilized for the determination of Terbinafine HCl (TRH). A 150 mm × 4.6 mm i.d., Shim-pack Cyanopropyl-bonded stationary phase was used for analysis. A mixture of 0.15 M sodium dodecyl sulphate (SDS), 0.3% triethylamine(TEA), 10% n-propanol all prepared in 0.02 M orthophosphoric acid was the mobile phase. The pH of the mobile phase was adjusted to 5.5 and pumped at a flow rate of 1 mL/min. The column temperature was set at 45°C. A fluorescence detection of (TRH) at 336 nm emission λ after excitation at 236 nm. Chlorzoxazone (CLZ) was used as an internal standard (IS). The method was linear over the range of 0.4-8.0 μg/mL. The limit of detection (LOD) and limit of quantification (LOQ) were 0.07 μg/ mL and 0.22 μg/mL, respectively. The method was further applied for analysis of the studied.. View More»
DOI:
10.4172/2157-7064.1000388