Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Edery Marc
France
Research Article
Medaka Proteome Study: Use of Cross-species Matching and Expressed Sequence Tag Data for Protein Identification
Author(s): Mezhoud Karim, Praseuth Danièle, Marie Arul, Puiseux-Dao Simone and Edery MarcMezhoud Karim, Praseuth Danièle, Marie Arul, Puiseux-Dao Simone and Edery Marc
Proteomics aims to understand gene function and molecular processes of the living cell through a large-scale study of the expressed proteins. Although proteomics approach is largely applied on experimental models such as rodents, other animal models such as medaka fish (Oryzias latipes) also attracts considerable interest in proteomics field. Medaka is one of the most studied animal model in reproductive or developmental biology. Although, its genome has been sequenced, only a few proteins are available in the databases. We present the procedure used in one experiment as a model of identification of proteins, in this case from liver of medaka treated by a hepatotoxic cyanotoxin, the microcystin. Because O. latipes is not listed in mascot search engine, the identification of proteins (selected because modified by the treatment) was done on closed relat.. View More»
DOI:
10.4172/jpb.1000063