Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
David M Lubman
1150 West Medical Center Drive, Building MSRB1 Rm A510B, Ann Arbor, MI 48109-0656
Tanzania
Research Article
Protein Markers Associated with an ALDH Sub-Population in Colorectal
Cancer
Author(s): Rui Yang, Xinhua Liu, Smathorn Thakolwiboon, Jianhui Zhu, Xiucong Pei, Mingrui An, Zhijing Tan and David M LubmanRui Yang, Xinhua Liu, Smathorn Thakolwiboon, Jianhui Zhu, Xiucong Pei, Mingrui An, Zhijing Tan and David M Lubman
ALDH has been shown to be a marker that denotes a sub-population of cancer stem cells in colorectal and other cancers. This sub-population of cells shows an increased risk for tumor initiation, metastasis, and resistance to chemotherapy and radiation resulting in recurrence and death. It is thus essential to identify the important signaling pathways related to ALDH1+ CSCs in colon cancer. The essential issue becomes to isolate pure sub-populations of cells from heterogeneous tissues for further analysis. To achieve this goal, tissues from colorectal cancer Stage III patients were immuno-stained with ALDH1 antibody. Target ALDH1+ and ALDH1- cells from the same tissue were micro-dissected using Laser Capture Microdissection (LCM). Captured cells were lysed and analyzed using LC-MS/MS where around 20,000 cells were available for analysis. This analysis r.. View More»
DOI:
10.4172/jpb.1000412