Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
David K Crossman
Tanzania
Research Article
Analysis of the Human Proteome in Subcutaneous and Visceral Fat Depots
in Diabetic and Non-diabetic Patients with Morbid Obesity
Author(s): Lingling Fang, Kyoko Kojima, Lihua Zhou, David K Crossman, James A Mobley and Jayleen GramsLingling Fang, Kyoko Kojima, Lihua Zhou, David K Crossman, James A Mobley and Jayleen Grams
No longer regarded as simply a storage depot, fat is a dynamic organ acting locally and systemically to modulate energy homeostasis, glucose sensitivity, insulin resistance, and inflammatory pathways. Here, mass spectrometry was used to survey the proteome of patient matched subcutaneous fat and visceral fat in 20 diabetic vs 22 nondiabetic patients with morbid obesity. A similar number of proteins (~600) were identified in each tissue type. When stratified by diabetic status, 19 and 41 proteins were found to be differentially abundant in subcutaneous fat and omentum, respectively. These proteins represent pathways known to be involved in metabolism. Five of these proteins were differentially abundant in both fat depots: moesin, 78 kDa glucose-regulated protein, protein cordon-bleu, zinc finger protein 611, and cytochrome c oxidase subunit 6B1. Three proteins, decorin, cytochrome.. View More»
DOI:
10.4172/jpb.1000361