Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Daisuke Higo
Japan
Research Article
Applying Magnetic Bead Separation / MALDI-TOF Mass Spectrometry to Human Tear Fluid Proteome Analysis
Author(s): Eiichi Sekiyama, Yumiko Matsuyama, Daisuke Higo, Takashi Nirasawa, Masaya Ikegawa, Shigeru Kinoshita and Kei TashiroEiichi Sekiyama, Yumiko Matsuyama, Daisuke Higo, Takashi Nirasawa, Masaya Ikegawa, Shigeru Kinoshita and Kei Tashiro
The proteins and peptides in tears play an important role in preserving the integrity and stability of the ocular surface. Proteomic analysis of tear films will enable us to detect early biological markers of eye diseases, however, it is often hampered by the small amount of tear volume and the low protein concentration. Here we adopted magnetic bead-based purification (ClinProt system) followed by matrix-assisted laser desorption/ionization timeof- flight mass spectrometry (MALDI-TOF-MS) to profile human tear proteins. Basal and reflex tear fluids were collected from normal healthy volunteers using glass microcapillary tubes. Reversed phase (C8) and weak cation exchange (WCX) magnetic beads were applied to obtain multiple components detected as clear signals. Principal component analysis showed a clear differentiation between basal and reflex tears. Among the key alt.. View More»
DOI:
10.4172/jpb.1000045