Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Dacheng He
IR Iran
Research Article
Gel-based Proteomic Characterization of Soluble and Insoluble Fraction Proteins in Rat Spinal Cord
Author(s): Shuguang Yang, Qinxue Ding, Yaojun Guo, Congjian Zhao, Yufeng Jia, Haiping Que, Hongxia Wang, Kaihua Wei, Dacheng He, Shuqian Jing and Shaojun LiuShuguang Yang, Qinxue Ding, Yaojun Guo, Congjian Zhao, Yufeng Jia, Haiping Que, Hongxia Wang, Kaihua Wei, Dacheng He, Shuqian Jing and Shaojun Liu
Fractionation efficiency and protein characterization of neural soluble and insoluble proteins by sequential extraction was scrutinized by gel-based proteomic analysis. Spinal cord proteins of adult rats were first extracted with aqueous buffer (fraction A), followed by standard (fraction B) or modified (fraction C) enhanced solutions. Of the top 30 most abundant proteins in fractions A, B and C, the percentage of cytoplasmic proteins was 74% (28/38) , 37% (14/38) and 42% (15/36), respectively; membrane organellar proteins accounted for 8% (3/38), 45% (17/38), and 44% (16/36); membrane proteins accounted for 13% (5/38), 18% (7/38) and 14% (5/36). The number of hydrophobic domains was 5, 15 and 9. Shared proteins in three fractions were only 13%. When additional less abundant 30 spots enriched the insoluble fraction C were characterized, membrane proteins accounted for.. View More»
DOI:
10.4172/jpb.1000124