Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Claudio Nicolini
Via Antonio Pastore 3, Genoa 16132
Italy
Research Article
Ab Initio Semi-Quantitative Analysis of Micro-Beam Grazing-Incidence
Small-Angle X-Ray Scattering (M-GISAXS) during Protein Crystal Nucleation
and Growth
Author(s): Claudio Nicolini, Nicola Luigi Bragazzi, Eugenia Pechkova and Remi LazzariClaudio Nicolini, Nicola Luigi Bragazzi, Eugenia Pechkova and Remi Lazzari
Micro-beam Grazing-Incidence Small-Angle X-ray scattering (μ-GISAXS), exploiting both the advantages of elastic X-ray scattering and the highly focused third-generation synchrotron radiation micro-beams, is an advanced scattering technique that enables scientists to unravel the details of crystal growth processes and to investigate large-scale structures in thin films, including nanobiofilms or other different kinds of surfaces, such as surface gradients or confined surfaces. In this study, we analyze semi-quantitatively and we simulate our previously acquired μ-GISAXS experiments of Thaumatin and Lysozyme Langmuir-Blodgett (LB)-film, shedding light on nucleation and crystal growth processes. Here, we show that, during LB-thin film facilitated nucleation, the particle radius of Thaumatin and of Lysozyme crystal increases while the film thickness reduces. Structural re-organizati.. View More»
DOI:
10.4172/jpb.1000303