Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
B.P. Mohanty
India
Research Article
Identification of Milk Protein Polymorphism in Indian Goats by 2D Gel Electrophoresis
Author(s): Ajay Kumar, P.K. Rout and B.P. MohantyAjay Kumar, P.K. Rout and B.P. Mohanty
Analysis of casein and whey protein was carried out in six Indian goats by both SDS-PAGE and 2-DE analysis. The variation was observed mainly in αs1, αs2, β, and κ-casein and β-lacto globulin locus in these breeds. Proteome analysis showed the presence of high number of spots in αs1-casein, and κ-casein showed highest number of spots in all the goat breeds. The gels were showing remarkably both variability and similarity suggesting that the heterogeneity in protein forms in individual milk samples exist, and milk protein represents a common pattern of post-translational modification.
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DOI:
10.4172/jpb.1000252