Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Beutel S
Institute of Technical Chemistry,
Callinstr 5, 30167 Hannover
Germany
Research Article
Evaluation of Driving Forces for Protein Partition in PEG-Salt Aqueous Two- Phase Systems and Optimization by Design of Experiments
Author(s): Glyk A, Solle D, Scheper T and Beutel SGlyk A, Solle D, Scheper T and Beutel S
In the present study, the partitioning of four model proteins (bovine serum albumin, ovalbumin, α-chymotrypsin and lysozyme) was investigated in polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPS) addressing the effects of PEG molecular weight (MW) and concentration, phase-forming salt type (potassium phosphate/sodium citrate (KPP/NaCit)) and concentration, sodium chloride (NaCl) concentration and pH, as well as MW, isoelectric point, charge and hydrophobicity of the proteins. It was found that protein partitioning was influenced by different coexisting effects, such as hydrophobic and electrostatic interactions, related to the system parameters and physicochemical/surface properties of proteins. Moreover, a rigorous analysis and optimization of the significant forces for protein partitioning in PEG-salt ATPS was performed by design of experiments (DoE). Initially, K.. View More»
DOI:
10.4172/2157-7064.1000389