Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Bernd Thiede
Norway
Research Article
A Proteomic Approach to Screening of Dynamic Changes in Detergent-Resistant Membranes from Activated Human Primary T Cells
Author(s): Kristine Moltu, Elisa Bjørgo, Therese Solstad, Torunn Berge, Bernd Thiede and Kjetil TaskénKristine Moltu, Elisa Bjørgo, Therese Solstad, Torunn Berge, Bernd Thiede and Kjetil Taskén
Upon stimulation of the T cell receptor, proteins involved in proximal T cell signaling gather in lipid rafts to guide external stimuli to the intracellular compartment through the formation of signaling complexes. The rearrangement of lipid rafts upon engagement of the T cell receptor is crucial to ensure a rapid and efficient signal transduction. To assess lipid raft protein composition, we here provide a qualitative mass spectrometric characterization of the Detergent- Resistant Membrane (DRM) proteome in human primary T cells, identifying a total of 425 proteins. Furthermore, we have addressed proteins solely associated with DRMs in resting T cells and proteins solely observed in DRMs upon engagement of the T cell receptor. Only a small group of proteins were found to be exclusively located to DRMs under either of these conditions, indicating that the protein composition of DRMs i.. View More»
DOI:
10.4172/jpb.1000264