Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Benjamín Fernández-Gutiérrez
HospitalClínico San Carlos, C/ Profesor Martín Lagos s/n,
28040 Madrid
Spain
Research Article
Differential Proteome of Articular Chondrocytes From Patients with Osteoarthritis
Author(s): Raquel Rollín, Pilar Tornero, F ernando Mar co, Emilio Camafeita, Enrique Calvo, Luis López-Durán, Juan Ángel Jover, Juan Antonio López, José Ramón Lamas and Benjamín Fernández-GutiérrezRaquel Rollín, Pilar Tornero, F ernando Mar co, Emilio Camafeita, Enrique Calvo, Luis López-Durán, Juan Ángel Jover, Juan Antonio López, José Ramón Lamas and Benjamín Fernández-Gutiérrez
Cartilage damage is a major problem in osteoarthritis (OA). To gain insight into the pathogenesis of OA, we have analyzed the differential proteome of articular chondrocytes from these patients. Protein extracts were prepared from cultured chondrocytes from 6 patients with end-stage OA and 6 normal donors and were analyzed by 2D-DIGE. Differentially expressed proteins were identified by mass spectrometry (MS). Significant differential expression was observed for 27 proteins, with 14 underexpressed and 13 overexpressed chondrocyte OA proteins. Of special interest was the identification of destrin, cofilins, gelsolin, annexin A2, glycolytic enzymes, chaperones, cathepsin D, proteasome beta 9 subunit isoform 2 proprotein and proteasome activator hPA28 subunit beta. The altered expression of these proteins is consistent with events such as cytoskeleton binding, protein di.. View More»
DOI:
10.4172/jpb.1000034