Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Barna Vásárhelyi
Nagyvárad tér 4.1089 Budapest
Hungary
Research Article
Validation of Biomarkers in Gene Expression Datasets of Inflammatory
Bowel Disease: IL13RA2, PTGS2 and WNT5A as Predictors of
Responsiveness to Infliximab Therapy
Author(s): András Gyorffy, Máté Kormos, Luca Bartha, András Szabó, Balázs Gyorffy, Jan Budczies and Barna VásárhelyiAndrás Gyorffy, Máté Kormos, Luca Bartha, András Szabó, Balázs Gyorffy, Jan Budczies and Barna Vásárhelyi
Background: Some patients with inflammatory bowel disease (IBD) do not respond to infliximab (IFX) therapy. Gene expression studies revealed genes that may help to predict non-responding IBD patients. Our purpose was to validate the discriminating power of published genes.
Methods: Microarray datasets of IBD patients responding or non-responding to IFX were downloaded from GEO database (‘transcriptomic arm’). Published genes discriminating responding and non-responding patients were identified in PubMed (‘biomarker arm’). Using ROC-analysis, the discriminating performance of genes in ‘biomarker arm’ were re-tested in each datasets of ‘transcriptomic arm’. We also performed an independent analysis of colon biopsy datasets to identify novel discriminating genes.
Results: The transcriptomic arm of four GEO datasets (3 and 1 .. View More»
DOI:
10.4172/jpb.1000329