Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Attaya Suvannasankha
Indiana University School of Medicine, and Richard L. Roudebush VA Medical Center,
Indianapolis, IN 46202
Tanzania
Research Article
Proteomic Characterization of Plasma Cells from Patients with Multiple Myeloma
Author(s): Attaya Suvannasankha, Colin D. Crean, Heather M. Leyes, Sariya Wongsaengsak, Guihong Qi, Jong-Won Kim and Mu WangAttaya Suvannasankha, Colin D. Crean, Heather M. Leyes, Sariya Wongsaengsak, Guihong Qi, Jong-Won Kim and Mu Wang
Introduction: Quantitative proteomics approaches have provided insight into biomarkers of cancer and other diseases with high sensitivity, high specificity, and high analytical precision. Multiple Myeloma is an incurable, fatal blood cancers characterized by clonal expansion of plasma cells in the bone marrow. Current multiple myeloma proteomic research mainly focuses on serum biomarkers, not plasma cells, due to technical difficulties including a requirement for tumor cell isolation from bone marrow aspirates, tumor cell paucity and poor in vitro survival after isolation.
Materials and methods: A global proteomic analysis was performed using sorted bone marrow plasma cells from normal donors and multiple myeloma patients and a large-scale quantitative mass spectrometry platform. A selected panel of up- and down-regulated proteins were validate.. View More»
DOI:
10.4172/jpb.1000461