Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Annika Thorsell
Institute of Neuroscience and Physiology,
Mölndal
Sweden
Research Article
Proteome Analysis of Serum-Containing Conditioned Medium from Primary Astrocyte Cultures
Author(s): Annika Thorsell, Jonas Faijerson, Fredrik Blomstrand, Michael Nilsson, Kaj Blennow, Peter S Eriksson and Ann Westman-BrinkmalmAnnika Thorsell, Jonas Faijerson, Fredrik Blomstrand, Michael Nilsson, Kaj Blennow, Peter S Eriksson and Ann Westman-Brinkmalm
Development of strategies that allow for identification of secreted factors in conditioned media is of significance for a wide range of research areas. Secreted factors are involved in intercellular communication and might also be biomarkers of potential clinical importance, used for early detection and diagnosis of disease. The aim of this study was to investigate whether metabolic labeling combined with mass spectrometry could be used to identify secreted proteins in serum-containing conditioned medium. Earlier proteomic studies of conditioned media have been performed on cells cultured in serum-free media. In the present study the fact that only the proteins derived from the cells contain the incorporated isotopically labeled amino acid was taken advantage of, making it possible to differentiate released proteins from medium proteins. The second objective was to ex.. View More»
DOI:
10.4172/jpb.1000019