Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

+44 1223 790975

Alexander J. R. Bishop

Alexander J. R. Bishop
Greehey Children’s Cancer Research Institute,

  • Research Article
    P53 and HIS-tag Binding
    Author(s): Lindsey Barron and Alexander J. R. BishopLindsey Barron and Alexander J. R. Bishop

    P53 is a globular protein with distinct domains and a key tumor suppressor that functions through transcriptional transactivation, repression and protein-protein interactions. Numerous studies have implicated protein-protein interactions between p53 and a multitude of cellular proteins with a variety of known functions. Because of these interactions, and the many gene expression regulations, a multitude of potential mechanisms and their relationship to tumor suppression have been proposed. It is desirable to test these interactions in an in vitro setting to demonstrate that any identified interaction is direct. Due to the difficulties associated with purifying recombinant full-length p53, many studies have utilized the p53 DNA binding domain to test for direct protein interactions with p53. The DNA binding domain of p53 is structured, folds independently and dictates the stability of .. View More»
    DOI: 10.4172/jpb.1000467

    Abstract PDF

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