Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Abhijit Chakrabarti
Saha Institute of Nuclear Physics,
1/AF, Bidhannagar, Kolkata-700064
India
Research Article
Fractional Precipitation of Plasma Proteome by Ammonium Sulphate:Case Studies in Leukemia and Thalassemia
Author(s): Sutapa Saha, Suchismita Halder, Dipankar Bhattacharya, Debasis Banerjee and Abhijit ChakrabartiSutapa Saha, Suchismita Halder, Dipankar Bhattacharya, Debasis Banerjee and Abhijit Chakrabarti
Human plasma proteome is a comprehensive source of disease biomarkers. However, the >10 orders-wide dynamic concentration range of its constituent proteins necessitates depletion of abundant proteins from plasma prior to biomarker discovery. Our objective has been to develop a simple method that would deplete the most abundant proteins e.g. albumin and immunoglobulins, effectively facilitating identification of differentially regulated proteins in plasma samples. We employed ammonium sulphate based pre-fractionation of plasma followed by twodimensional gel electrophoresis (2DGE) for comparison of normal proteins with those from the plasma samples of the patients, after identification of proteins by MALDI-TOF/TOF tandem mass spectrometry. Fractional precipitation of the plasma samples by 20% ammonium sulphate from raw plasma doubled the number of pr.. View More»
DOI:
10.4172/jpb.1000230