The main objective of this study was to produce fermented probiotic drink with the help of Lactobacillus plantarum. The fermentation was carried at 37°C for 72 hr under aerobic condition. The parameters like pH, microbial load, antioxidant activity (DPPH), acidity & concentration of reducing sugar had been measured. The antibacterial against E.coli, Staphylococcus and pseudomonas was determined. Fermented juice was subjected cytotoxic activity against some cancer cell line & it showed activity. The presence of various types of organic acids was determined by HPLC analysis.