Purpose: In order to produce new functional seafood, rainbow trout were acclimation in seawater and fed with inclusion of CLA and carotenoid extract from sea squirt tunic. Lipid extract from the sea-reared rainbow trout muscle (RME) and viscera (RVE) were administered to rats simultaneously with normal or high fat diet for six weeks. We evaluated the effects of the dietary supplementation with CLA, carotenoids, and n-3 HUFA by the beneficial effects of anti-obesity activities.
Materials and method: Sea-reared rainbow trout (800 ± 20.5 g; 40.0 ± 1.7 cm) were fed with basal feed which supplemented with 10 mg/kg sea squirt tunic’s carotenoids and 3% CLA (w/w) for three months. Lipid was extracted from rainbow trout muscle and viscera using chloroform/methanol (2/1). Forty eight male Sprague Dawley rats (400 ± 2.0 g) were randomly divided into six groups (n=8). Two types of diets, normal types (SN, RVN, RMN) and 45% of high fat research diets (SHF, RVHF, RMHF) were used. The excised liver and epididymal adipose tissues were fixed in 3% formaldehyde solution, embedded in paraffin. Organs sections were stained with hematoxylin-esosin. Histological examination on liver and adipose tissues sections were carried out with a light microscope.
Results: Sea farming and inclusion of CLA and carotenoids pigment extracted from the tunic of sea squirt, the content of C18:1n-9 was reduced to 32.5% in the muscle’s lipid and 35.8% in the viscera’s lipid. Serum lipid and liver enzymes activity in blood serum showed that RME and RVE have the liver protective ability.
Conclusion: RME and RVE have potency as weight reducer through fat burner routes as well as liver protective ability. Hence, it could be considered as anti-obesity supplement for the long period of administration.