Journal of Leukemia
Open Access
ISSN: 2329-6917
+44 1300 500008
ISSN: 2329-6917
+44 1300 500008
Xiaoyan Chen
IR Iran
Research Article
Beta-Hydroxyisovalerylshikonin Inhibits the Growth of U266 Multiple Myeloma Cells by Triggering the Mitochondrial Pathway
Author(s): Yihui He, Xiaoyan Chen and Lingying KongYihui He, Xiaoyan Chen and Lingying Kong
Background: Beta-hydroxyisovalerylshikonin (beta-HIVS) is a compound isolated from the traditional oriental medicinal herb lithospermum radix. This drug exerts a role as an ATP non-competitive inhibitor of Protein-Tyrosine Kinases (PTKs) and shows great potential for induction of apoptosis against human cancer cells. We investigated the effect of beta-HIVS on multiple myeloma U266 cells and clarified details of the primary mechanism in its apoptosisinducing activity. Objective: The aim of this work was to trace the apoptosis-inducing activity of beta-HIVS on U266 cells as well as the underlying mechanisms. Methods: Cell Counting Kit-8 (CCK-8) test and colony-forming assay were performed in estimating the effects of beta-HIVS on U266 cell viability and colony formation. Apoptosis analysis was carried out on the basis of DAPI fluorescence staining and DNA fragmentation assays. Real-time PC.. View More»
DOI:
10.4172/2329-6917.1000155