Journal of Drug Metabolism & Toxicology
Open Access
ISSN: 2157-7609
+44-20-4587-4809
ISSN: 2157-7609
+44-20-4587-4809
Xianglin Duan
Professor, The Key Labortry of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province,
Hebei Normal University, Shijiazhuang, 050024, Hebei Province,
Bolivarian Rep. of Venezuela
Research Article
Effect of Glutamate on Brain Iron Metabolism and the Regulation Mechanism
Author(s): Peng Yu, Ming Zhang, Hui Ding, Xiaojing Di, Peng Guan, Shumin Wang, Zhenhua Shi, Dongyun Jiang, Xianglin Duan and Yan-Zhong ChangPeng Yu, Ming Zhang, Hui Ding, Xiaojing Di, Peng Guan, Shumin Wang, Zhenhua Shi, Dongyun Jiang, Xianglin Duan and Yan-Zhong Chang
Glutamate is an excitatory transmitter and can induce neurotoxicity, it can also increase the iron concentrations in the brain, but little is known about the detailed molecular regulation mechanism of iron metabolism by Glu. Based on our previous data, iron metabolism related proteins might be associated with the increase of brain iron contents induced by neurotransmitter. To investigate the issues, the iron contents, non-transferrin-bound iron (NTBI) uptake and the expression of iron uptake and iron release proteins were firstly examined in vivo and in vitro with iron histochemistry, inductively coupled plasma mass spectroscopy (ICP-MS), 55Fe radioactive liquid scintillation counting and western blot methods. Data showed that glutamate induced the increase of total iron contents, storage iron contents and NTBI uptake activity. Moreover, only divalent metal transporter 1, one of iron .. View More»
DOI:
10.4172/2157-7609.1000190