Journal of Drug Metabolism & Toxicology
Open Access

Nobunao Ikewaki

Publications

• Research Article
  A Mechanism by Which Propofol Induces Cytotoxicity
  Author(s): Shin Onizuka, Nobunao Ikewaki and Seiji ShiraishiShin Onizuka, Nobunao Ikewaki and Seiji Shiraishi
  
  Background: Many studies have reported on the cytotoxicity of propofol; however, the mechanism is still unclear. The aim of this study was to clarify the mechanisms of cytotoxicity induced by propofol. Methods: Using Hela cells, viability was measured using a calcein-AM assay. Apoptosis was observed by DNA fragmentation, caspase activity. Mitochondrial membrane potential, and mitochondrial pH were measured using the ratiometric fluorescent probes JC-1 and SNARF-1 to clarify the mechanisms of mitochondrial cytotoxicity. Furthermore, the pH of both the intra- and extra-membrane was measured simultaneously using 5-hexadecanoylaminofluorescein (HAF), a ratiometric pH sensitive fluorescent probe. Results: Propofol (0~69.6 μM) reduced the viability of Hela cells in a dose- and time-dependent manner. Propofol induced .. View More»
  DOI: 10.4172/2157-7609.1000230

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