Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
+44 1223 790975
ISSN: 2155-9570
+44 1223 790975
Mei Tsuda
Kansai Medical University Takii Hospital,
10-15 Fumizono-cho, Moriguchi, Osaka 570-8507
Japan
Research Article
Controllable Urokinase Gene Expression in Trabecular Meshwork Cells
Author(s): Mei Tsuda, Shiho Kaneko, Akira Ando, Tetsuya Nishimura, Emiko Okuda-Ashitaka, Seiji Ito, Makoto Taomoto, Miyo Matsumura and Kanji TakahashiMei Tsuda, Shiho Kaneko, Akira Ando, Tetsuya Nishimura, Emiko Okuda-Ashitaka, Seiji Ito, Makoto Taomoto, Miyo Matsumura and Kanji Takahashi
Purpose: Glaucoma is a type of progressive optic neuropathy that finally leads to blindness related to elevated intraocular pressure (IOP). Accumulation of extracellular matrix in trabecular meshwork (TM) and juxta-canalicular connective tissues, which form an aqueous outflow pathway, may be a major cause of increased IOP, thus the fibrinolytic system may be associated with regulation of IOP. We examined the possibility of controllable urokinase plasminogen activator (uPA) gene transfer into TM cells.Methods: TM cells were freshly isolated from porcine eyes and human TM cells obtained during trabeculectomy procedures and cultured. Total RNA was extracted from human TM cells and reverse transcribed into cDNA. A reverse-transcribed polymerase chain reaction (RT-PCR) method was then performed to detect the gene expression of uPA. The cDNA of human .. View More»
DOI:
10.4172/2155-9570.1000329