ISSN: 2471-9315
Kosuke Kasai
Japan
Research Article
The Effects of Pantoea and Kosakonia Isolated from Buckwheat Sprouts on Obese Mice.
Author(s): Kanako Yamanouchi, Yamato Sakamoto, Miyuki Fujioka, Kosuke Kasai, Takakiyo Tsujiguchi, Tashimitu Kimura, Manabu Shimazu, Akira Kato and Koichi ItoKanako Yamanouchi, Yamato Sakamoto, Miyuki Fujioka, Kosuke Kasai, Takakiyo Tsujiguchi, Tashimitu Kimura, Manabu Shimazu, Akira Kato and Koichi Ito
Objective: This study aims to investigate the effects of surface bacteria of buckwheat sprouts on obesity. We examine whether these bacteria have probiotic properties.
Methods: Since P-36 and P-37 strains were present in large amounts on the buckwheat sprouts surface, identification of species was carried out by intestinal bacteria identification kit and 16S rRNA gene analysis. Subsequently, to investigate the biological effects, P-36 and P-37 strains were divided into two groups, heat-treated dead bacteria and untreated viable bacteria, which were then orally administered to obese model mice exhibiting hyperglycemia every other day for a total of 10 times. After administration, BMI and visceral fat mass were measured, and oral glucose tolerance test was conducted to evaluate glucose tolerance; their influence on blood glucose level and insu.. View More»
DOI:
10.4172/2471-9315.1000146