Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
+44 1223 790975
ISSN: 2155-9570
+44 1223 790975
Katharina L. Neuer
Klinik und Poliklinik für Augenheilkunde Klinikum rechts der Isar, Technische,
Munich
Germany
Research Article
In Vitro Biocompatibility of Silicone Oil Siluron Xtra® on Porcine Retina in a Perfusion Culture System
Author(s): Katharina L. Neuer, Sabrina Bohnacker, Nikolaus Feucht, Chris P. Lohmann and Mathias MaierKatharina L. Neuer, Sabrina Bohnacker, Nikolaus Feucht, Chris P. Lohmann and Mathias Maier
Purpose: To examine the biocompatibility of Siluron Xtra® (Fluoron, Ulm, Germany), a silicone oil used as a vitreous substitute in vitreo-retinal surgery on porcine retinal tissue in vitro.
Methods: Retinal tissue of 37 porcine eyes was immediately prepared post mortem and placed into Minucell perfusion systems (Minucell, Bad Abbach, Germany) to be perfused with liquid culture medium over a period of 4-8 days. 23 retinal tissue samples including retinal pigment epithelium (RPE) were covered by silicone oil (group 1) during the perfusion and compared to a control group of 7 samples (group 2) without silicone oil. Group 3 included 7 single RPE tissues without retinal tissue layer in direct contact with silicone oil, thus imitating a retinal tear, during the perfusion.
The morphology of the retina and RPE was examined by light mic.. View More»
DOI:
10.4172/2155-9570.1000679