Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
Dong-Woo Cho
77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk, 790-784
Research Article
Human Turbinate-derived Mesenchymal Stem Cells Differentiated into Keratocyte Progenitor Cells
Author(s): Moon Nyeo Park, Bonglee Kim, Hyeonji Kim, Sun Hwa Park, Mi-Hyun Lim, Yeong-jin Choi, Hee-Gyeong Yi, Jinah Jang, Sung Won Kim and Dong-Woo ChoMoon Nyeo Park, Bonglee Kim, Hyeonji Kim, Sun Hwa Park, Mi-Hyun Lim, Yeong-jin Choi, Hee-Gyeong Yi, Jinah Jang, Sung Won Kim and Dong-Woo Cho
Objective: Though keratoplasty is used to treat corneal blindness, donor shortage, tendency of stimulated keratocyte transformed to fibroblast and immunological rejection are still big problems. As a solution, cornea tissue engineering using non-corneal tissue sourced cells become emerging issue. Thus, this study was designed to find novel material for keratoplasty. Methods: Human turbinate-derived mesenchymal stem cells (hTMSCs) were obtained from patients and cultured with differentiation medium for 14 days. The keratocyte markers, stem cell markers, early corneal stromal stem cell (CSSC) markers, were measured by real time-PCR. The MSC markers were detected by FACS. Results: After 14 days of differentiation medium exposure, hTMSCs expressed markers of keratocyte such as keratocan sulfate proteoglycan (KERA) and aldehydrogenase (ALDH). As the hTMSCs became keratocytes, the expressio.. View More»
DOI:
10.4172/2155-9570.1000627