Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
David Shealy
China
Research Article
Blockade of Tumor Necrosis Factor Alpha Prevents Complications of Diabetic Retinopathy
Author(s): Hu Huang, Wen Li, Jianbo He, Patricia Barnabie, David Shealy and Stanley A VinoresHu Huang, Wen Li, Jianbo He, Patricia Barnabie, David Shealy and Stanley A Vinores
Tumor necrosis factor alpha (TNFα) plays an important role in the pathogenesis of diabetic retinopathy (DR). The objective of this study is to investigate the effect of TNFα blockade on complications of DR. Experimental models of diabetes were induced with streptozotocin (STZ) injection or insulin 2 gene point mutation (Akita) in mice. Intravitreal (IVT) and intraperitoneal (IP) injections were used to deliver anti-TNFα antibody and saline control. TUNEL and activated caspase-3 staining were used to examine apoptotic cell death. Transcardially-perfused FITC-ConA and fluorescence microscopy were used to monitor leukocyte adhesion. Trypsin digestion was used to prepare retinal vasculature and quantify acellular capillary. The leakage of 3H-mannitol into the retina was used to quantify the breakdown of blood-retinal barrier (BRB). TNFα blockade significantly prevented.. View More»
DOI:
10.4172/2155-9570.1000384