Journal of Clinical and Experimental Ophthalmology
Open Access

Danielle Béland

Publications

• Research Article
  Amplified Chromatographic Signal and UV-Absorbance Spectroscopy to Evaluate Lysozyme after Thermal Denaturation and in Contact Lens Extracts
  Author(s): Claude J Giasson, Danielle Béland and Langis Michaud Claude J Giasson, Danielle Béland and Langis Michaud
  
  Objective: Amplify the absorbance signal related to the lysozyme extracted from contact lenses as measured by high-performance liquid chromatography (HPLC); examine the purity of lysozyme and how much thermal denaturation affects its elution profile. Methods: In trial chromatographic runs during which contact lens extracts had been injected into the system, the fractions collected between 4 and 5.5 minutes pointed to lysozyme as the eluting protein as indicated by a Western blot. Proteins were extracted from contact lenses in a 50:50 solution of 0.2% trifluoroacetic acid (TFA): acetonitrile (ACN). Each contact lens extract was separated into 2 aliquots. After vacuum evaporation, aliquots no 1 were dissolved into the initial mobile phase to produce an enrichment factor of 8. Aliquots no 2 were left untreated in the regular extraction solution. C.. View More»
  DOI: 10.4172/2155-9570.1000596

  Abstract PDF

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