Journal of Drug Metabolism & Toxicology
Open Access
ISSN: 2157-7609
+44-20-4587-4809
ISSN: 2157-7609
+44-20-4587-4809
Bidhan Chandra Koner
Maulana Azad Medical College,
2 BSZ Marg, New Delhi 110002
India
Research Article
The Effect of Chlorpyrifos, an Organophosphorus Pesticide, on Glucose Uptake in Whole Blood
Author(s): Shrijana Shrestha, Vijay Kumar Singh, Shanmugasundaram B, Sajib Kumar Sarkar, Jeevaratnam K and Bidhan Chandra KonerShrijana Shrestha, Vijay Kumar Singh, Shanmugasundaram B, Sajib Kumar Sarkar, Jeevaratnam K and Bidhan Chandra Koner
Background: The sub-chronic organophosphorous pesticide exposure increases insulin resistance and thereby the risk of developing Diabetes mellitus. Its acute exposure also leads to hyperglycemia. However, the mechanism is not clearly understood. Hence the present study explores the effect of low and high concentrations of chlorpyrifos, an organophosphorous pesticide on glucose uptake by whole blood and its possible mechanism. Methods: Level of hemolysis was determined at 2 and 4 hours of 1% DMSO (Dimethyl sulfoxide) and chlorpyrifos (0.3 and 10 mg/ml) exposure. The whole blood was treated in vitro with vehicle i.e., 1% DMSO or high (10 mg/mL) or low (0.3 mg/mL) concentrations of chlorpyrifos dissolved in DMSO. Then plasma glucose was assayed by glucose oxidase method at 2 and 4 hours of DMSO/chlorpyrifos exposures. Results: There was no effect of DMSO or 0.3 mg/ml chlorpyrifos treatment .. View More»
DOI:
10.4172/2157-7609.1000211