Journal of Nutrition & Food Sciences
Open Access
ISSN: 2155-9600
+32 25889658
ISSN: 2155-9600
+32 25889658
Lu Ma, Fang Wang, Girma Debele Delelesse, Toddy R Callaway, Dengpan Bu*
Overproduction of free radicals during oxidation of free fatty acids and deficiency of antioxidants caused oxidative stress and metabolic disorders. Reactive oxygen species can initiate lipid peroxidation because lipids are prone to oxidation. Carnitine is a methylated form of lysine that acts as a potent antioxidant that counteracts the occurrence of oxidative stress. However, the interaction of high energy diet and rumen protected lysine in dairy cows has not been explored well. The objective of the current study was to investigate the effect of pre-calving dietary energy levels with or without bypass lysine on malondialdehyde concentrations and antioxidant status in dairy cows during the transition period. Forty 3rd lactation Holstein cows were randomly allocated to one of the four dietary treatments (HEByls=1.53 NEL plus 40 g bypass Lysine, HECK=1.53 NEL without bypass Lys, LEbyls=1.37 NEL plus 40 g bypass Lysine and LECK=1.37 NEL without bypass Lysine) arranged in a 2 × 2 factorial design. Blood samples were collected at day -21, -14, -7, 7, 14, 21 relative to calving and malondialdehyde concentrations, glutathione peroxidase activity, superoxide dismutase activity and total antioxidant capacity were analyzed using assay kits. The results indicated that a pre-calving high-energy diet significantly increased glutathione peroxidase activity and reduced malondialdehyde concentrations in prepartum cows. Rumen protected lysine significantly decreased malondialdehyde concentrations and increased total antioxidant capacity in postpartum cows. It was concluded that a high-energy diet and rumen protected lysine substantially improved antioxidant status and reduced malondialdehyde concentrations in dairy cows during the transition period.
Published Date: 2025-04-09; Received Date: 2023-11-20