Journal of Nutrition & Food Sciences

Journal of Nutrition & Food Sciences
Open Access

ISSN: 2155-9600

+44 7480022449


Development and Application of an LC-MS/MS Method for Determining Methylsulfonylmethane (MSM) levels in Human Plasma

Ling Lin*, Dejian Ma, Richard J. Bloomer, Matthew Butawan, Webb A. Smith, Charles R. Yates

Methylsulfonylmethane (MSM) isan organosulfur phytochemical widely used as a dietary supplement carrying structure/function claims that includethe promotion of joint health. Untargeted metabolomics studies using nuclear magnetic resonance (NMR) have identified MSM in plasma and urine. However, MS-based methodology is more suitable for pharmacokinetic studies designed to explore MSM’s concentration-effect relationship for purposes of establishing dosing guidelines. To address this deficiency, an LC-MS/MS method using MSM, deuterated MSM (MSM-d6, internal standard), and liquid-liquid extraction was developed and validated in accordance with international guidelines. The method proved well-suited for determining MSM levels in human plasma following chronic oral administration (1, 2, or 3 grams daily) for four weeks. The availability of a robust LC-MS/MS method and MSM dose linearity data provides a framework for exploring MSM’s concentration-effect relationship to support various structure/function claims.

Published Date: 2020-05-11; Received Date: 2020-04-15