Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

P K Sahoo

Department of Pharmaceutics, Delhi Pharmaceutical Sciences and Research University, India

  • Research Article   
    Exploring the Concept of Hydrotrophy in HPLC; Hydrotrophic Solutions as Novel Cost Effective, Eco-friendly and safe Mobile Phase for Method development and validation of Furosemide
    Author(s): Ahammed unais V P* and P K Sahoo

    Hydrotrophy is a unique molecular phenomenon that possess the power to extend the solubility of sparingly soluble and poorly soluble drugs in water. A hydrotrophic solution is defined as adding a solute, that is Hydrotrope, (eg: urea, Nicotinamide, Sodium benzoate, Sodium citrate etc.) to the solvent and is used as mobile phase for RP-HPLC. This concept minimizes the use of harmful and costly oragnic solvents to a larger extend which makes drugs analysis ecofriendly and less expensive. The methods was developed using 3% sodium benzoate (pH 5.5) as single mobile phase to review and conclude this subject. Welch ultisil ODS-3(C18) coloumn is used as stationary phase. The flow rate was maintaibed at 1 ml/min and detection was performed at 233 nm using DAD detector. The retension time was 4.5 min and method was detected to be linear over a concentration range of 25-100 microgram/ml with co.. View More»
    DOI: 10.35248/2157-7064.21.12.450

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