Journal of Hematology & Thromboembolic Diseases
Open Access
ISSN: 2329-8790
ISSN: 2329-8790
Zhao Wen-Li
China
Research Article
CDH13 is Frequently Inactivated by Promoter Hypermethylation in Pediatric Acute Myeloid Leukemia (AML)
Author(s): Tao Yan-Fang, Feng Xing, Wang Jian, Zhao Wen-Li, Xiao-Juan Du, Wu Shui-Yan, Wang Na, Hu Shao-Yan, Cao Lan, Li Yan-Hong, Ni Jian and Pan JianTao Yan-Fang, Feng Xing, Wang Jian, Zhao Wen-Li, Xiao-Juan Du, Wu Shui-Yan, Wang Na, Hu Shao-Yan, Cao Lan, Li Yan-Hong, Ni Jian and Pan Jian
There is growing evidence supporting a role for tumor suppressor as targets in aberrant mechanisms of DNA hypermethylation. Methylation in the promoter of tumor suppressor always plays important roles in pediatric Acute Myeloid Leukemia (AML). CDH13 gene is a tumor suppressor involved in tumorigenesis, metastasis and apoptosis in a variety of tumors. In this study, we are trying to investigate whether CDH13 was down regulated by promoter methylation in pediatric AML. MRNA transcriptional expression levels of CDH13 were evaluated by semi-quantitative PCR and real-time PCR. Methylation status of CDH13 prompter was investigated by Methylation Specific PCR (MSP) and Bisulfate Genomic Sequencing (BGS). CDH13 mRNA transcription was inactivated in AML cell lines. Promoter of CDH13 was aberrantly methylated in 55.6% (5/9) leukemia cell lines. Promoter aberrant methylation of CDH13 was detected i.. View More»
DOI:
10.4172/2329-8790.1000111