Journal of Plant Biochemistry & Physiology
Open Access
ISSN: 2329-9029
+44 7868 792050
ISSN: 2329-9029
+44 7868 792050
Yin H
China
Research Article
Transcriptome Analysis of Tessellated and Green Leaves in Paphiopedilum Orchids Using Illumina Paired-End Sequencing and Discovery Simple
Sequence Repeat Markers
Author(s): Li D, Yin H, Zhao C, Zhu G and Lu FLi D, Yin H, Zhao C, Zhu G and Lu F
Transciptome analysis based on next generation sequencing allows quantitative comparisons of gene expression across diverse species. Using Illumina sequencing, we generated a total of 35.7 and 30.1 million paired-end reads with lengths of 100 bp from Paphiopedilum concolor tessellated leaves and Paphiopedilum hirsutissimum green leaves, respectively. De novo assembly yielded 68,602 and 54,273 unigenes with average lengths of 844 and 874 bp for each species leaves, respectively. Based on BLAST searches with known protein sequences, 46.6% unigenes from P. concolor and 48.6% unigenes from P. hirsutissimum were annotated. Gene ontology, cluster of orthologous groups and kyoto encyclopedia of genes and genomes annotations revealed that the functions of the transcripts from the two Paphiopedilum species leaves covered a similarly broad set of molecular functions, biological.. View More»
DOI:
10.4172/2329-9029.1000136