Mass Spectrometry & Purification Techniques

Mass Spectrometry & Purification Techniques
Open Access

ISSN: 2469-9861


Toshihiko Hanai

Toshihiko Hanai
Research Institute for Production Development 4F,
Sakyo-ku, Kyoto 606-0805

  • Review Article
    Hydrophilic Interaction Liquid Chromatography for LC-MS
    Author(s): Toshihiko HanaiToshihiko Hanai

    The retention mechanisms in hydrophilic interaction liquid chromatography (HILIC) are quantitatively described in in silico analysis. Among a variety of polar bonded-phase silica gels, hexylamine-bonded silica gel is stable and can be used in both aqueous HILIC and ion-exchange liquid chromatography with high reproducibility. Hexynyl and phenylhexyl bonded silica gels are also used in HILIC with high reproducibility. The retention mechanisms in aqueous hydrophilic and non-aqueous hydrophilic (normal-phase) liquid chromatography are similar. Both hydrogenbonding and weak electrostatic interaction contributes to retention. Furthermore, strong electrostatic interaction is dominant in ion-exchange liquid chromatography. A suitable liquid chromatographic system for liquid chromatography (LC)-mass spectrophotometer (MS) is proposed... Read More»
    DOI: 10.4172/2469-9861.1000123

    Abstract PDF

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