Mass Spectrometry & Purification Techniques
Open Access

Suzanne M de la Monte

Publications

• Research Article
  Comparative Analysis of Lipid Extracts and Imaging Mass Spectrometry for Evaluating Cerebral White Matter Biochemical Pathology in an Experimental Second-Hand Cigarette Smoke Exposure Model
  Author(s): Alexander Krotow, Emine B Yalcin, Jared Kay and Suzanne M de la Monte Alexander Krotow, Emine B Yalcin, Jared Kay and Suzanne M de la Monte
  
  Background: White matter injury and degeneration are common features of developmental and aging-associated diseases, yet their pathobiological bases are poorly understood. However, recent advances in Matrix-Assisted Laser Desorption Ionization (MALDI) instruments and chemistry have provided critical tools for myelin-lipid analytical research. Design: This study characterizes Cigarette Smoke (CS) exposure effects on frontal lobe lipid ion profiles in adult male A/J mice that had been exposed to air for 8 weeks (A8), CS for 4 (CS4) or 8 weeks (CS8), or CS8 followed by 2 weeks recovery (CS8+R). MALDI data acquired by analysis of lipid extracts plated onto a ground steel target (high through-put) were compared with Imaging Mass Spectrometry (IMS). Results: MALDI-time-of-flight (TOF) detected 120 lipid ions with m/z?s of 600 to 1300 (phospholipids and sulfatides) in samples plated onto the st.. View More»
  DOI: 10.4172/2469-9861.1000113

  Abstract PDF

Relevant Topics