Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
+44 1478 350008
ISSN: 2161-0495
+44 1478 350008
Suleiman Al-Hammadi
United Arab Emirates
Research Article
Impaired Forebrain Cellular Bioenergetics Following Acute Exposure to Ammonia
Author(s): Mohammed T. Alsamri, Suleiman Al-Hammadi, Sami Shaban, Aisha Alshamsi, GhazalaBelal Balhaj, Thachillath Pramathan and Abdul-Kader SouidMohammed T. Alsamri, Suleiman Al-Hammadi, Sami Shaban, Aisha Alshamsi, GhazalaBelal Balhaj, Thachillath Pramathan and Abdul-Kader Souid
Introduction: The main purpose of this study was to report on the acute neurotoxicity of ammonia, using cellular respiration and ATP as surrogate metabolic biomarkers. Cellular respiration (mitochondrial O2 consumption), ATP and glutathione (GSH) were measured in forebrain specimens after intraperitoneal administration of 3.8 to 28.8 μmol/g ammonia to Taylor Outbred mice; the lethal dose was ≥ 30 μmol/g. Methods: Cortical fragments were collected in phosphate-buffered saline plus 5 mM glucose and immediately processed for measuring O2 consumption, using a phosphorescence O2 analyzer. Cellular ATP was determined with the luciferase/luciferin system. Cellular GSH was labeled with monobromobimane and the bimane derivatives were separated on HPLC and detected by fluorescence. In the presence of the tissue specimen in a vial sealed from air, O2 concentration declined linearly with.. View More»
DOI:
10.4172/2161-0495.1000189