Cell & Developmental Biology
Open Access

Steven R Head

Publications

• Research Article
  Glycomic Signature of Mouse Embryonic Stem Cells During Differentiation
  Author(s): Rania Harfouche, Somak Ray, Melinda Sanchez, Ushashi Dadwal, Steven R Head, Aaron Goldman and Shiladitya Sengupta Rania Harfouche, Somak Ray, Melinda Sanchez, Ushashi Dadwal, Steven R Head, Aaron Goldman and Shiladitya Sengupta
  
  Background: The glycome has emerged as a key regulator of cell fate, partly through its ability to potentiate the action of numerous signaling pathways. We recently demonstrated that a sulfated component of the glycome plays a critical role in promoting the differentiation of embryonic stem cell (ESC)-derived embryoid bodies by modulating downstream growth factors, such as the insulin-like growth factor (IGF) signaling axis. However, the exact components of the glycome which promote ESC differentiation versus stemness remain uncharacterized, due to the lack of a rapid, simple and easily quantifiable methodology. As a proof-of-concept in this study, we utilized a custom-made glycoarray in combination with bioinformatics and molecular biology tools in order to uncover novel glyco-signatures underlying ESC differention in an embryoid body model. A better elucidation of the glycomic trans.. View More»
  DOI: 10.4172/2168-9296.1000120

  Abstract PDF

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