Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
+44 1478 350008
ISSN: 2161-0495
+44 1478 350008
Simon J Knight
Dstl, Porton Down, Salisbury, Wiltshire
United Kingdom
Research Article
Evaluation by ELISA of Ricin Concentration in Fluids and Tissues after Exposure to Aerosolised Ricin, and Evaluation of an
Immunochromatographic Test for Field Diagnosis
Author(s): Gareth D Griffiths, Simon J Knight, Jane L Holley and Philippe ThullierGareth D Griffiths, Simon J Knight, Jane L Holley and Philippe Thullier
Introduction: We have formerly developed a point-of-care Immunochromatographic Test (ICT) for the diagnosis of ricin pulmonary intoxication, which was intended to be utilised after nasal swabbing. The requested limit of sensitivity for such a diagnosis was calculated but not tested experimentally, and no other sample source was considered. Here, this approach was evaluated on various samples taken from mice intoxicated by a low dose of aerosolised ricin, to push the system to its limits. The best source of sampling for the diagnosis of ricin intoxication was assessed, as was the validity of the ICT.
Methods: Mice were intoxicated by 3LCt50 of aerosolised ricin and bodily fluids (nasal and lung lavages, blood and urine) were sampled until the 24th hour after intoxication to evaluate their ricin content, using a sensitive ELISA. The ricin concentrations in solid tissues (lungs, .. View More»
DOI:
10.4172/2161-0495.1000162