Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
+44 1300 500008
ISSN: 2157-7013
+44 1300 500008
Shu-Hsia Chen
Tanzania
Research Article
Combined Inhibition of Epidermal Growth Factor Receptor and Cyclooxygenase-2 as a Novel Approach to Enhance Radiotherapy
Author(s): Shibo Fu, Michael Rivera, Eric C Ko, Andrew G. Sikora, Chien-Ting Chen, Ha Linh Vu, David Cannan, Samuel Eisenstein, Barry S. Rosenstein, Julio Aguirre- Ghiso, Shu-Hsia Chen and Johnny KaoShibo Fu, Michael Rivera, Eric C Ko, Andrew G. Sikora, Chien-Ting Chen, Ha Linh Vu, David Cannan, Samuel Eisenstein, Barry S. Rosenstein, Julio Aguirre- Ghiso, Shu-Hsia Chen and Johnny Kao
Targeting epidermal growth factor receptor (EGFR) is a promising approach to increasing radiosensitivity of head and neck cancers but treatment resistance remains an important clinical problem. We hypothesize that combined EGFR and cyclooxygenase-2 (COX-2) inhibition, using small molecule inhibitors erlotinib and celecoxib, respectively would further increase the antitumor activity of radiotherapy. The effects of combinations of celecoxib, erlotinib and ionizing radiation (IR) on cell growth, cell cycle progression and apoptosis of head and neck cancer cell lines were assessed in vitro by cell viability, clonogenic survival, flow cytometry and Annexin V assays and in vivo. The effects of celecoxib, erlotinib and IR on primary and downstream molecular targets were analyzed by immu.. View More»
DOI:
10.4172/2157-7013.S1-002