Pancreatic Disorders & Therapy
Open Access

Shinichi Takano

Publications

• Research Article
  Alterations in Cancer-related Genes Associated with Grading of Well Differentiated Pancreatic Neuroendocrine Neoplasms
  Author(s): Yudai Yokota, Mitsuharu Fukasawa, Shinichi Takano, Hiroko Shindo, Ei Takahashi, Makoto Kadokura, Kunio Mochizuki, Shinya Maekawa, Jun Itakura, Hideki Fujii, Tadashi Sato, Nobuyuki Enomoto Yudai Yokota, Mitsuharu Fukasawa, Shinichi Takano, Hiroko Shindo, Ei Takahashi, Makoto Kadokura, Kunio Mochizuki, Shinya Maekawa, Jun Itakura, Hideki Fujii, Tadashi Sato, Nobuyuki Enomoto
  
  Objectives: Although recent advances in next-generation sequencing (NGS) have revealed some genetic alterations in various tumors, including pancreatic neuroendocrine tumors (PanNETs), their clinical significance is not fully understood. To investigate the clinical significance of gene alteration in PanNETs, we performed genetic analysis of well differentiated PanNETs using NGS. Methods: Twenty-nine resected primary PanNET tissue samples and three samples of metastatic liver tissues, obtained from 29 PanNET patients, were analyzed. DNA was extracted from laser-captured formalin-fixed paraffinembedded tissues, and 50 cancer-associated genes, including approximately 2,800 hotspots, were amplified by multiplex PCR. Amplified libraries were sequenced using NGS, and the results were analyzed in conjunction with respective clinicopathological feat.. View More»
  DOI: 10.4172/2165-7092.1000189

  Abstract PDF

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