Pancreatic Disorders & Therapy
Open Access
ISSN: 2165-7092
+44 7868 792050
ISSN: 2165-7092
+44 7868 792050
Shinichi Takano
Faculty of Medicine,
1110, Shimokato, Chuo, Yamanashi 409-3898
Japan
Research Article
Alterations in Cancer-related Genes Associated with Grading of Well Differentiated Pancreatic Neuroendocrine Neoplasms
Author(s): Yudai Yokota, Mitsuharu Fukasawa, Shinichi Takano, Hiroko Shindo, Ei Takahashi, Makoto Kadokura, Kunio Mochizuki, Shinya Maekawa, Jun Itakura, Hideki Fujii, Tadashi Sato and Nobuyuki EnomotoYudai Yokota, Mitsuharu Fukasawa, Shinichi Takano, Hiroko Shindo, Ei Takahashi, Makoto Kadokura, Kunio Mochizuki, Shinya Maekawa, Jun Itakura, Hideki Fujii, Tadashi Sato and Nobuyuki Enomoto
Objectives: Although recent advances in next-generation sequencing (NGS) have revealed some genetic alterations in various tumors, including pancreatic neuroendocrine tumors (PanNETs), their clinical significance is not fully understood. To investigate the clinical significance of gene alteration in PanNETs, we performed genetic analysis of well differentiated PanNETs using NGS.
Methods: Twenty-nine resected primary PanNET tissue samples and three samples of metastatic liver tissues, obtained from 29 PanNET patients, were analyzed. DNA was extracted from laser-captured formalin-fixed paraffinembedded tissues, and 50 cancer-associated genes, including approximately 2,800 hotspots, were amplified by multiplex PCR. Amplified libraries were sequenced using NGS, and the results were analyzed in conjunction with respective clinicopathological feat.. View More»
DOI:
10.4172/2165-7092.1000189