Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
ISSN: 2161-0495
Santokh Gill
Health Canada, Regulatory Toxicology Research Division, Health Products and Food Branch, Tunney’s Pasture,
Health, 251 Sir Frederick Banting Driveway, Ottawa, ON, K1A 0K9
Canada
Research Article
Serum Proteomic Analysis for the Identification of Biomarkers by Two-
Dimensional Differential Gel Electrophoresis (2D-DIGE) after Exposure to the
Food-Processed Contaminant Furan
Author(s): Santokh Gill, Meghan Kavanagh, Christine Poirier, Dorcas Weber and Terry KoernerSantokh Gill, Meghan Kavanagh, Christine Poirier, Dorcas Weber and Terry Koerner
Background: Furan is a compound known to be present in cooked or thermally-processed foods by formation through traditional heat treatment processes and also known to be a potent hepatotoxin. To identify potential biomarkers of furan exposure, two-dimensional differential gel electrophoresis (2D-DIGE) was performed on individual serum samples to identify proteomic profiles that were differentially expressed in rats exposed to furan at 0, 0.03, 0.5 and 8.0 mg/kg bw/day. Results: There were no differences in protein expression between control and the 0.03 and 0.5 mg/kg bw/day dose groups (one-way ANOVA P<0.05, avg ratio +1.5). At the 8.0 mg/kg bw/day of furan exposure, there were 22 protein spots that showed a difference in expression when compared to the control animals (one-way ANOVA P<0.05, avg ratio +1.5). After manual spot picking, four proteins were identified by standard in.. View More»
DOI:
10.4172/2161-0495.1000301