Anesthesia & Clinical Research
Open Access
ISSN: 2155-6148
ISSN: 2155-6148
Ralf Weißhorn
Germany
Research Article
In-Vitro and In-Vivo Effects of the Local Anaesthetic Dibucaine on Malignant Hyperthermia - Susceptible and Normal Swine
Author(s): Mark U. Gerbershagen, Ralf Weißhorn, Kerstin Kolodzie, Frank Wappler and Marko FiegeMark U. Gerbershagen, Ralf Weißhorn, Kerstin Kolodzie, Frank Wappler and Marko Fiege
Background: Ten case reports have described clinical signs and symptoms of malignant hyperthermia (MH) that developed following local anaesthetics. In this study, the In-Vitro effects of dibucaine, an amide local anaesthetic, were compared in skeletal-muscle samples from MH-susceptible (MHS) and normal (MHN) swine. In addition, we investigated the In-Vivo MH trigger potency of dibucaine in MHS swine. Methods: Trigger-free general anaesthesia was induced in six MHS and six MHN swine. Muscle biopsies were obtained. In the In-Vivo experiment, cumulative doses of dibucaine (1, 2, 4, 8, 16, and 32 mg/kg) were administered i.v. In the In-Vitro contracture test (IVCT), dibucaine was added cumulatively to muscle specimens from eight MHS and eight MHN muscles (bath concentrations: 0.1, 0.2, 0.4, 0.6, and 1.0 mmol/l). Results: In the In-Vivo test, no signs or symptoms of MH were observed. In-Vi.. View More»
DOI:
10.4172/2155-6148.1000172