Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
+44 1300 500008
ISSN: 2157-7013
+44 1300 500008
Punit Kaur
Biochemistry and Immunology, Morehouse School of Medicine,
720 Westview Drive SW, Atlanta, GA 30310
Tanzania
Research Article
Loss of Biofilm Formation in an Emerging Foodborne Pathogen
Enteroaggregative Escherichia coli (EAEC) Under Acid Stress
Author(s): Punit Kaur and Alexzander AseaPunit Kaur and Alexzander Asea
Purpose: Enteroaggregative Escherichia coli (EAEC) is an emerging foodborne pathogen as a significant diarrheal pathogen in multiple population groups. However, the associated phenotypic changes by which EAEC bacteria survive acid conditions, as it passes through the human digestive track, is not completely understood.
Materials and methods: EAEC (T8) was grown in vitro by mimicking the in vivo pH conditions found in human stomach and intestine.
Results: The lowest pH where EAEC (T8) was able to grow was pH 4.0 in Luria Bertani (LB) media, albeit with a lower growth rate and the bacteria reached log phase in approximately 7h. However, there was no significant difference in the growth pattern at mild acidic pH 5.5. In addition, EAEC (T8) grown at pH 4.0, demonstrated an absence of biofilm formation, clump or pellicl.. View More»
DOI:
10.4172/2157-7013.1000260