Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
+44 1300 500008
ISSN: 2157-7013
+44 1300 500008
Pramod Kumar Gautam
All India Institute of Medical Science,
3rd floor, PC block, New Delhi 110029
India
Research Article
Biologically Synthesized Gold Nanoparticles using Ocimum sanctum (Tulsi Leaf Extract) Induced Anti-Tumor Response in a T Cell Daltons Lymphoma
Author(s): Pramod Kumar Gautam, Sanjay Kumar, Tomar MS, Rishi Kant Singh, Acharya A, Ritis Shyanti K, Anita, Sonal Swaroop, Sanjay Kumar and Ram BPramod Kumar Gautam, Sanjay Kumar, Tomar MS, Rishi Kant Singh, Acharya A, Ritis Shyanti K, Anita, Sonal Swaroop, Sanjay Kumar and Ram B
The characterizations of green synthesized gold nanoparticles (AuNPs) prepared using Ocimum sanctum leaf extract were done by UV-Spectrophotometer at 500-540 nm. The XRD data obtained were found similar to gold JCPDS File No- 04-0784. SEM and TEM analysis of AuNPs revealed spherical shape and size of 200 nm. Further FT-IR data indicated the various biomolecules present in Ocimum sanctum leaf extract provides stability to gold nanoparticles synthesis. The AuNPs were studied for their anti-cancer activity on Dalton’s lymphoma (DL) cells and the results obtained with IC50 value of < 50 ng/ml performed by MTT assay. Further, to confirm anti-tumor potential and the mode of action of the synthesized AuNPs, cell viability assay, nuclear morphology, DNA fragmentation assay, mitochondrial membrane potential (ΔΨm) analysis, and cell cycle analysis were done using DL cells. DL.. View More»
DOI:
10.4172/2157-7013.1000278