Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
+44 1300 500008
ISSN: 2157-7013
+44 1300 500008
Pramod C Rath
School of Life Sciences,
New Delhi 110067
India
Research Article
A Simple Method for Isolation, Propagation, Characterization, and
Differentiation of Adult Mouse Bone Marrow-Derived Multipotent
Mesenchymal Stem Cells
Author(s): Jitendra Kumar Chaudhary and Pramod C RathJitendra Kumar Chaudhary and Pramod C Rath
Mesenchymal stem cells (MSCs) are spindle-shaped, adherent, clonogenic, non-phagocytic, fibroblastic and multipotent in nature with the intrinsic ability of self-renewal and proliferation. Bone marrow is the richest source of MSCs for studying the underlying processes of proliferation, self-renewal, and multiple-lineage differentiation. There have been several studies to improve the method of isolation, propagation, characterization and differentiation of mesenchymal stem cells from the mouse bone marrow, but none are widely acceptable. Owing to unavailability of universally acceptable method of MSCs culture continuous efforts are being made in this direction. Here, we report a simple method with some subtle modifications aiming to improve the overall method of isolation, culture, propagation and differentiation of MSCs in vitro. Following this protocol, we have isolated MSCs with typ.. View More»
DOI:
10.4172/2157-7013.1000261