Journal of Plant Biochemistry & Physiology
Open Access
ISSN: 2329-9029
ISSN: 2329-9029
Peters W
Germany
Research Article
Characterization of a Glycan Exo-Hydrolase that Shows a Biphasic Expression
in the Course of an In Vitro Culture of Photoautotrophic Oxybasis rubra
Cells
Author(s): Dominguez OV, Peters W, Reinbothe C, Schwarz W, Zverlov V and Beck EDominguez OV, Peters W, Reinbothe C, Schwarz W, Zverlov V and Beck E
Like plant leaves, suspension cultures of photoautotrophic Oxybasis rubra Fuentes-Bazan (syn. Chenopodium rubrum L.) cells pass through distinct developmental phases when grown under CO2 as the sole carbon source: an initial cell division phase of 4 weeks, a stationary phase of another 4 weeks and an aging phase (3-4 weeks) when the cell senesce and finally die. These phases are reflected by differential gene expression. A gene that was strongly expressed in the course of the stationary phase but much lesser during the exponential growth phase of the cell culture was isolated from a cDNA-library of stationary cells and completed by 5’-RACE. From homology analysis, the gene was tentatively identified as glycan exo-hydrolase (Oxybasis rubra glycan exo-hydrolase, OrGEH). Heterologous expression in E. coli yielded a protein with a preference to hydrolyze the ß.. View More»
DOI:
10.4172/2329-9029.1000137