Journal of Plant Biochemistry & Physiology
Open Access
ISSN: 2329-9029
+44 1478 350008
ISSN: 2329-9029
+44 1478 350008
Nzaro G Makenzi
PO Box 90420, Mombasa
Kenya
Research Article
In Vitro Plant Regeneration of Sweetpotato Through Direct Shoot Organogenesis
Author(s): Nzaro G Makenzi, Wilton M Mbinda, Richard Oduor Okoth and Mathews Piero NgugiNzaro G Makenzi, Wilton M Mbinda, Richard Oduor Okoth and Mathews Piero Ngugi
Sweetpotato (Ipomoea batata) is an important, nutritionally rich vegetable crop, but severely affected by environmental stresses, pests and diseases which cause massive yield and quality losses. Genetic manipulation is becoming an important method for sweetpotato improvement. In the present study, a reproducible and highly efficient protocol for in vitro plant regeneration of six Kenyan farmer preferred sweetpotato, Enaironi, KEMB 36, KSP36, Mugande, Kalamb nyerere, SPK 013 and SPK004 through direct shoot organogenesis from stem internodes explants was developed. The results revealed that Kalamb nyerere had the highest number of adventitious bud; for light (5.33 and 4.33) and dark (8.00 and 5.00) induction condition for all TDZ hormone level (0.25 mg/l and 0.15 mg/l). When explants incubated in 0.10 mg/l NAA the regeneration frequencies were the highest at 83.33% (Jewel) and 96.67% (K.. View More»
DOI:
10.4172/2329-9029.1000207